El propósito de mis investigaciones ha sido estudiar los mecanismos celulares y moleculares por los cuales las hormonas esteroides regulan la función neuronal. Un aspecto importante ha sido explorar la regulación de los comportamientos reproductivos que se presentan durante el estro de las ratas (conducta de lordosis y proceptivas) por medio de las hormonas ováricas, el estradiol (E2) y la progesterona (P). Queremos entender cómo la modulación hormonal de la transmisión sináptica en regiones específicas del cerebro coordina la expresión de la receptividad sexual femenina (lordosis). Nuestro trabajo demuestra que la regulación hormonal de la lordosis se produce a través de la modulación de diferentes vías de comunicación intracelular inducida por una variedad de agentes con diferentes estructuras químicas como; progestinas, GnRH, PGE2, AMPc y por aspectos sensoriales que se producen por la estimulación vagino cervical. Por lo menos dos mecanismos distintos de acción de la hormona se han identificado: 1) un mecanismo membranal, que implica la activación de diferentes proteínas cinasas que provocan la fosoforilación de otras proteínas y) un mecanismo genómico en donde participa el receptor intracelular de la progesterona. Sin embargo hoy se conoce que ambos mecanismos pueden tener una comunicación cruzada para generar el efecto fisiológico final. Además, estos trabajos también se han encaminado a explorar la participación del sistema del óxido nítrico sobre la conducta de estro inducido por los agentes antes descritos. Estos trabajos han mostrado que diferentes compuestos lordogénicos son capaces de activar la conducta de estro a través de la modulación de este sistema, así como la participación del GMP cíclico y su proteína cinasa dependiente.





The objective of my investigation has been to study cellular and molecular mechanisms by which steroid hormones regulate neuronal function. One important aspect has been to explore the regulation of reproductive behaviors that are stimulated during estrus in female rats (lordosis and proceptive behavior), by the ovarian hormones estradiol (E2) and progesterone (P). We aim to understand how the hormonal modulation of synaptic transmission in specific regions of the brain coordinate the expression of female sexual receptivity (lordosis). Our work demonstrates that the hormonal regulation of lordosis occurs via the modulation of of different intracellular messenger pathways, induced by a variety of agents with different chemical structures such as: progestins, GnRH, PGE2, cAMP, as well as by sensory stimuli associated with vaginal cervical stimulation. At least two distinct mechanisms of hormonal action have been identified: 1) a cell membrane mechanism that involves the activation of different protein kinases that in turn phosphorylate other proteins, and 2) a genomic mechanism in which the intracellular P receptor participates. However, it is now known that both mechanisms can have a cross-communication to generate the final physiological effect. Moreover, these investigations have begun to explore the participation of the nitric oxide system in estrous behavior induced by the aforementioned agents. This work has shown that different lordogenic agents have the capacity to activate estrous behavior through the modulation of this system, as well as the participation of cGMP and its target protein kinases.
Dr. Óscar González Flores
Laboratorio de Neuroendocrinología Celular y Molecular, Centro de Investigación en Reproducción Animal, UATx.

oglezflo@hotmail.com
Doctorado / Doctorate: Biología de la Reproducción, UATx

Perfil PROMEP

SNI Nivel II
Socio regular de la Sociedad Mexicana de Ciencias Fisiológicas, A.C.

Socio regular de la Society for Neuroscience (SFN).

Socio regular de la Society for Behavioral Neuroendocrinology.

Miembro de la Academia de Investigación en Biología de la Reproducción, A.C.
2018. Camacho-Arroyo I, González-Arenas A, Jiménez-Arellano C, Morimoto S, Galván-Rosas A, Gómora-Arrati P, García-Juárez M, González-Flores O.Sex hormone levels and expression of their receptors in lactating and lactating pregnant rats. J Steroid Biochem Mol Biol. 178:213-220.

2018. Domínguez-Ordóñez R, Garcia-Juárez M, Lima-Hernández FJ, Gómora-Arrati P, Domínguez-Salazar E, Blaustein JD, Etgen AM, González-Flores O. Lordosis facilitated by GPER-1 receptor activation involves GnRH-1, progestin and estrogen receptors in estrogen-primed rats.
Horm Behav.98:77-87.

2016. Portillo W, González-Flores O, Camacho FJ, Mani SK, Paredes RG. Participation of progesterone receptors in facilitation and sequential inhibition of lordosis response induced by ring A-reduced progesterone metabolites in female mice. Behav Neurosci. 130(6):624-34.

2016. Domínguez-Ordóñez R, García-Juárez M, Lima-Hernández FJ, Gómora-Arrati P, Blaustein JD, Etgen AM, González-Flores O.Estrogen receptor a and ß are involved in the activation of lordosis behavior in estradiol-primed rats. Horm Behav. 86:1-7.

2016. Kubli-Garfias C, Lima-Hernández FJ, Vázquez-Ramírez R, Gómora-Arrati P, García-Juárez M, González-Flores O. The importance of the chemical structure of pregnanes in the concurrent inhibition of estrous behavior in the female rat.J Steroid Biochem Mol Biol. 163:51-8.

2016. Eguibar JR, Cortes C, Toriz CG, Romero-Carbente JC, González-Flores O, Fernández-Guasti A. Differential organization of male copulatory patterns in high- and low-yawning-frequency sublines versus outbred Sprague-Dawley rats. Physiol Behav Vol. 1 Pag. 84-84

2016. Enrique Basurto, Oscar González Flores Kurt Leroy Hoffman. Glycinamide prevents MK-801-induced hyperactivity and deficits in objectrecognition memory in
an animal model ofpositive and cognitive symptomsof schizophrenia, Schizophrenia Research Vol. 166 Pag. 349-349

  1. Domínguez-Ordóñez R, García-Juárez M, Lima-
Hernández FJ, Gómora-Arrati P, Blaustein JD, González-Flores O. Sexual receptivity facilitated by unesterified estradiol: Dependence on estrogen and progestin
receptors and priming dose of estradiol benzoate, Behav Neurosci Vol. 129 Pag.
 
2015. Luna F, Barrientos E, Alatriste V, Martínez I, Limón ID, González-Flores O. Morphology and Chemical Phenotype of the Ovarian Intrinsic Neurons in Neonate and Sexually Mature Reproductive Guinea Pig. Advances in Reproductive Sciences 3: 13-26

2014. Lima-Hernández FJ, Gómora-Arrati P, García-Juárez M, Blaustein JD, Etgen AM, Beyer C, González-Flores O. Estrogen receptors regulate the estrous behavior induced by progestins, peptides, and prostaglandin E2. Horm Behav. 66: 361-368.

2014. González-Arenas A, Piña-Medina AG, González-Flores O, Galván-Rosas A, Porfirio Gómora-Arrati, Camacho-Arroyo I. Sex hormones and expression pattern of cytoskeletal proteins in the rat brain throughout pregnancy. J Steroid Biochem Mol Biol. 139:154-8.

2013. García-Juárez M, Beyer C, Gómora-Arrati P, Domínguez-Ordoñez R, Lima-Hernández FJ, Eguibar JR, Galicia-Aguas YL, Etgen AM, González-Flores O. Lordosis facilitation by leptin in ovariectomized, estrogen-primed rats requires simultaneous or sequential activation of several protein kinase pathways. Pharmacol Biochem Behav. 110:13-8.

2013. Beyer C, Komisaruk BK, González-Flores O, Gómora-Arrati P. Glycinamide, a glycine pro-drug, induces antinociception by intraperitoneal or oral ingestion in ovariectomized rats. Life Sci. 92(10):576-81.

2013. Kubli-Garfias C, González-Flores O, Gómora-Arrati P, González-Mariscal G, Vázquez-Ramírez R, Beyer C. Bimodal binding and free energy of the progesterone receptor in the induction of female sexual receptivity by progesterone and synthetic progestins. J Steroid Biochem Mol Biol. 133:43-50.

2013. Alatriste V, Herrera-Camacho I, Martínez MI, Limón ID., González-Flores O, Luna F. Sensory denervation with capsaicin reduces ovarian follicular development and delays the onset of puberty in guinea pigs. Advances in Reproductive Sciences. 3: 1-9.
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